Coding

Part:BBa_K3206002:Design

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


ChaC gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 135
    Illegal AgeI site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This basic part was made compatible with Type IIs restriction: sequence contains AATG and GCTT fusion sites for the 5' end and 3' end respectively.


Source

Escherichia coli K12, the source of the sequence was retrieved from UniProt- https://www.uniprot.org/uniprot/P39163

References

Kaur, A. et al., 2017. ChaC2, an Enzyme for Slow Turnover of Cytosolic Glutathione. Journal Of Biological Chemistry, 292(2), pp.638–651.